Anti-Insulin抗体[8E2] (HRP) (ab24455)


  • 产品名称Anti-Insulin抗体[8E2] (HRP)
    参阅全部 Insulin 一抗
  • 描述
    小鼠单克隆抗体[8E2] to Insulin (HRP)
  • 偶联物HRP
  • 特异性Kass for this antibody is 6.0 x 108M-1. It cross-reacts with human proinsulin. No cross-reaction with free C-peptide.
  • 经测试应用适用于: Sandwich ELISA, ELISAmore details
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Full length native protein (purified) (Human)



Our Abpromise guarantee covers the use of ab24455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [3A6] to Insulin (ab1965). Can be used as Detection antibody with recommended pair.
ELISA Use at an assay dependent dilution.


  • 功能Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 疾病相关Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Anti-Insulin antibody [8E2] (HRP) (ab24455)参考文献

ab24455 has not yet been referenced specifically in any publications.

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Merci d'avoir pris le temps de répondre à notre questionnaire.

Je suis désolé de ne pas avoir pu résoudre correctement le problème que vous avez rencontré avec l'anticorps anti-insuline ab24455...

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Thank you for contacting us.

In our we recommend carbonate/bicarbonate buffer at pH 9.6. However, the capture antibody can also be diluted with PBS, pH 7.4, and the HRP-conjuga...

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Application ELISA
Sample Mouse Purified protein (Insulin standards)
Specification Insulin standards
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 37°C
Type Sandwich (Detection)

Abcam user community

Verified customer

提交于 Oct 10 2012

Thank you for your reply.
Please keep in mind that mouse has not been tried and we do not know if and how well the antibodies will work with this species.
I'll pass the lot numbers to the lab.

I hope this information is helpful to you....

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Thank you for your phone call.
I have contacted the lab for more information about antibody dilutions and for a detailed protocol, and will let you know when I hear back.
Below, please find the discount codes for trying these antibodies with ...

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For ab24455 DISCOUNT CODE: ***

For ab1965 DISCOUNT CODE: ***

Expiration date: December 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab1965 and ab24455 for useon mouse samples. Each code...

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Thank you for yoiru enquiry.

I can confirm that these two antibodies have been tested together in Sandwich ELISA system. The storage instructions can be found on the on-line product datasheets. We normally suggest our customers using the prod...

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Merci pour votre réponse.

J'ai mis en place l'envoi de 4 unités de ab24455. Le numéro de commande de remplacement gratuit de ce produit est *****. Vous recevrez prochainement un mail de confirmation comprenant les d&eacu...

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Je suis vraiment désolé que le dernier lot GR73820 ne soit pas, une fois de plus, de bonne qualité.

C'est malheureusement le seul lot que nous avons actuellement en stock. Je pensais en vous contactant la semaine derni&eg...

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Thank you for your response.

You have raised an excellent point that I will bring to our datasheet team. While we do list sandwich ELISA as a different application perhaps we could list the specific ELISA type as we do for IHC. In most cases ...

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