Anti-Insulin抗体[7F8] (ab8302)

概述

  • 产品名称Anti-Insulin抗体[7F8]
    参阅全部 Insulin 一抗
  • 描述
    小鼠单克隆抗体[7F8] to Insulin
  • 特异性Human insulin
  • 经测试应用适用于: IHC-P, IHC-Fr, ELISAmore details
  • 种属反应性
    与反应: Cow, Human, Pig
  • 免疫原

    Full length native protein (purified) (Human).

  • 常规说明Kass for clone 7F8 is 7.5 x 109M-1

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab8302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.

Use at an assay dependent dilution.

IHC-Fr Use at an assay dependent concentration.

Use at an assay dependent dilution.

 

 

 

ELISA Use at an assay dependent concentration.

Use at an assay dependent dilution. For detection of insulin in two-site enzyme immunoassay, this antibody can be used in conjunction with ab8303 (D4B8) This antibody recognises a different epitope than the anti-proinsulin ab8301 (3A1).

靶标

  • 功能Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 疾病相关Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IDDM antibody
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Anti-Insulin antibody [7F8] 图像

  • Ab8302 staining human normal pancreas tissue. Staining is localised to extracellular spaces.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Insulin antibody [7F8] (ab8302)参考文献

ab8302 has not yet been referenced specifically in any publications.

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The antibodies you have chosen are absolutely fine. Please select any as per your requirements. These are all guaranteed for IHC-Fr which means if they don't work we will either be replacing them or will offer you refu...

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Clin Chem. 1994 Apr;40(4):531-6. Related Articles, Links Different forms of insulin-like growth factor-binding protein-3 detected in serum and seminal plasma by immunofluorometric assay with monoclonal antibodies. Koistinen H, Seppala M, Koi...

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