Anti-Insulin抗体[2D11-H5] (ab49560)


  • 产品名称Anti-Insulin抗体[2D11-H5]
    参阅全部 Insulin 一抗
  • 描述
    小鼠单克隆抗体[2D11-H5] to Insulin
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Cow, Human, Pig
    不与反应: Mouse, Rat
  • 免疫原

    Human insulin conjugated to BSA

  • 阳性对照
    • Human pancreatic tissue (Beta cells of the islets of Langerhans). This antibody gave a positive result when used in the following formaldehyde fixed cell line: Panc-1.




Our Abpromise guarantee covers the use of ab49560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.


  • 功能Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 疾病相关Defects in INS are the cause of familial hyperproinsulinemia (FHPRI) [MIM:176730].
    Defects in INS are a cause of diabetes mellitus insulin-dependent type 2 (IDDM2) [MIM:125852]. IDDM2 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Defects in INS are a cause of diabetes mellitus permanent neonatal (PNDM) [MIM:606176]. PNDM is a rare form of diabetes distinct from childhood-onset autoimmune diabetes mellitus type 1. It is characterized by insulin-requiring hyperglycemia that is diagnosed within the first months of life. Permanent neonatal diabetes requires lifelong therapy.
    Defects in INS are a cause of maturity-onset diabetes of the young type 10 (MODY10) [MIM:613370]. MODY10 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IDDM1 antibody
    • IDDM2 antibody
    • ILPR antibody
    • ins antibody
    • INS_HUMAN antibody
    • Insulin A chain antibody
    • Insulin B chain antibody
    • IRDN antibody
    • MODY10 antibody
    • Preproinsulin antibody
    • Proinsulin antibody
    • Proinsulin precursor antibody
    see all

Anti-Insulin antibody [2D11-H5] 图像

  • ICC/IF image of ab49560 stained Panc-1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab49560 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Mouse gG (H+L) (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical staining of insulin-containing cells in human paraffin embedded pancreatic tissue using ab49560 at 1/75 dilution. 60 minutes primary antibody incubation at 25oC and standard ABC technique used. Note intense cytoplasmic staining of the beta cells of the islets of Langerhans and of the tumor cells (center).

Anti-Insulin antibody [2D11-H5] (ab49560)参考文献

ab49560 has not yet been referenced specifically in any publications.

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