概述

  • 产品名称Anti-INSM1抗体
    参阅全部 INSM1 一抗
  • 描述
    兔多克隆抗体to INSM1
  • 特异性Ab30940 recognises INSM1.
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide derived from residues 393 - 442 of human INSM1.

    .

  • 阳性对照
    • Fetal lung lysate.

性能

应用

Our Abpromise guarantee covers the use of ab30940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21609490Cells fixed with 4% PFA, treated with 0.5% Triton for 5 minutes at Room Temperature.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/7.8125e+006.

靶标

  • 相关性INSM1 may be associated with the transformation of neuroendocrine cells. It is found in several tumor cell lines of neuroendocrine origin including pheochromocytoma, medullary thyroid carcinoma, insulinoma, pituitary tumor, and small cell lung carcinoma. It is not found in any of the normal tissues tested.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • IA1 antibody
    • Insulinoma associated protein 1 antibody
    • Zinc finger protein IA1 antibody

Anti-INSM1 antibody 图像

  • Anti-INSM1 antibody (ab30940) at 0.5 µg/ml (in 5% skim milk / PBS buffer) + fetal lung lysate at 10 µg/ml

    Secondary
    HRP conjugated anti Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

Anti-INSM1 antibody (ab30940)参考文献

This product has been referenced in:
  • Kamakura M  et al. Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0. Virol J 8:257 (2011). ICC/IF, WB . Read more (PubMed: 21609490) »
  • Kamakura M  et al. Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0. Virol J 8:257 (2011). WB, ICC/IF . Read more (PubMed: 21609490) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brain 2 day-old mouse)
Loading amount 20 µg
Specification Brain 2 day-old mouse
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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提交于 Jun 19 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"