概述

  • 产品名称Anti-Insig1抗体
    参阅全部 Insig1 一抗
  • 描述
    兔多克隆抗体to Insig1
  • 特异性ab70784 recognizes ~30 kDa InSig1 and its precursor (~50 kDa).
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Cow, Human
  • 免疫原

    Synthetic peptide surrounding amino acid 248 of rat Insig1.

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab70784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 30, 50 kDa (predicted molecular weight: 30, 50 kDa).Can be blocked with Insig1 peptide (ab126817).

靶标

  • 功能Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.
  • 组织特异性Expressed in all tissues tested with highest expression in the liver.
  • 序列相似性Belongs to the INSIG family.
  • 翻译后修饰Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CL 6 antibody
    • CL6 antibody
    • INSI1_HUMAN antibody
    • INSIG 1 antibody
    • INSIG 1 membrane protein antibody
    • INSIG-1 antibody
    • insig1 antibody
    • INSIG1 membrane protein antibody
    • Insulin induced gene 1 antibody
    • Insulin induced gene 1 protein antibody
    • Insulin-induced gene 1 protein antibody
    • MGC1405 antibody
    see all

Anti-Insig1 antibody 图像

  • Anti-Insig1 antibody (ab70784) at 0.5 µg/ml + Jurkat cell lysate

    Predicted band size : 30, 50 kDa
    Observed band size : 30,50 kDa
  • ICC/IF image of ab70784 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70784, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab70784 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70784, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Insig1 antibody (ab70784)参考文献

This product has been referenced in:
  • Wagschal A  et al. Genome-wide identification of microRNAs regulating cholesterol and triglyceride homeostasis. Nat Med 21:1290-7 (2015). Read more (PubMed: 26501192) »
  • Barbero-Camps E  et al. Endoplasmic reticulum stress mediates amyloid ß neurotoxicity via mitochondrial cholesterol trafficking. Am J Pathol 184:2066-81 (2014). Mouse . Read more (PubMed: 24815354) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification HepG2 cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 29 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience th...

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Thank you for contacting us. I am sorry to hear that the replacement product which was sent did not work as it should have. Under the terms of our Abpromise I am more than happy to provide you with a free of charge replacement of this product wi...

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Thank you for calling Abcam.

I am sorry that you are experiencing problems with ab70784 in western blot. As we discussed on the telephone, I am sending you a new vial of the antibody as a free of charge replacement and you should have it on Tu...

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Thank you for your inquiry.

I can confirm that Insig1 (Insulin-induced gene 1 protein) is expressed in many tissues (http://www.ncbi.nlm.nih.gov/pubmed/12202038).

Jurkat cells endogenously express Insig1 which makes them a good (and...

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Thank you for your call yesterday and for your patience.



I have just heard from the lab, and the blocking peptide is available forthis antibody. I am currently working to have this peptide added to our catalog, and I will be in tou...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (hela)
Loading amount 1e+006 cells
Specification hela
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Apr 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"