Anti-Insig1抗体(ab112248)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to Insig1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Insig1.

  • 阳性对照
    • This antibody gave a positive signal in HepG2 whole cell lysates as well as the following tissue lysates: Human Liver; Human Brain; Rat Brain.

性能

应用

Our Abpromise guarantee covers the use of ab112248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

靶标

  • 功能
    Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Seems to regulate the ubiquitin-mediated proteasomal degradation of HMGCR. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.
  • 组织特异性
    Expressed in all tissues tested with highest expression in the liver.
  • 序列相似性
    Belongs to the INSIG family.
  • 翻译后修饰
    Ubiquitinated. Subsequent to sterol deprivation, the SCAP-SREBF2 complex becomes dissociated from INSIG1, is then ubiquitinated and degraded in proteasomes. Although ubiquitination is required for rapid INSIG1 degradation, it is not required for release of the SCAP-SREBP complex. Ubiquitinated by RNF139.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CL 6 antibody
    • CL6 antibody
    • INSI1_HUMAN antibody
    • INSIG 1 antibody
    • INSIG 1 membrane protein antibody
    • INSIG-1 antibody
    • insig1 antibody
    • INSIG1 membrane protein antibody
    • Insulin induced gene 1 antibody
    • Insulin induced gene 1 protein antibody
    • Insulin-induced gene 1 protein antibody
    • MGC1405 antibody
    see all

Anti-Insig1 antibody 图像

  • All lanes : Anti-Insig1 antibody (ab112248) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human brain tissue lysate - total protein (ab29466)
    Lane 4 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

实验方案

Anti-Insig1 antibody (ab112248)参考文献

ab112248 has not yet been referenced specifically in any publications.

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Thank you for sending the protocol details. A replacement will not be a problem. I will look over the options and have a suggestion for you tomorrow.

I see in our records that the issue you had with ab70784 was that it gave high background, ...

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The aa99 Ala versus Thr substitution you describe for your sequence will not be an issue, given that the immunogen lies within aa50 - 90 of the human sequence, UniProt O15503, http://www.uniprot.org/uniprot/O1550...

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