概述

  • 产品名称Anti-INPP5F抗体
    参阅全部 INPP5F 一抗
  • 描述
    兔多克隆抗体to INPP5F
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 591-857 of the Human INPP5F protein (Q01968).

  • 阳性对照
    • 293T and A431 cell lysates, A431 cells and Human Breast ca tissue.

性能

应用

Our Abpromise guarantee covers the use of ab125917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Detects a band of approximately 104 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. citrate buffer-10mM
ICC/IF 1/100 - 1/500.

靶标

  • 功能Converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. Also converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. May function in lysosomal membrane trafficking by regulating the specific pool of phosphatidylinositol 4,5-bisphosphate that is associated with lysosomes.
  • 组织特异性Brain, skeletal muscle, heart, kidney, lung, placenta and fibroblasts.
  • 疾病相关Defects in OCRL are the cause of Lowe oculocerebrorenal syndrome (OCRL) [MIM:309000]. It is an X-linked multisystem disorder affecting eyes, nervous system, and kidney. It is characterized by hydrophthalmia, cataract, mental retardation, vitamin D-resistant rickets, aminoaciduria, and reduced ammonia production by the kidney. Ocular abnormalities include cataract, glaucoma, microphthalmos, and decreased visual acuity. Developmental delay, hypotonia, behavior abnormalities, and areflexia are also present. Renal tubular involvement is characterized by impaired reabsorption of bicarbonate, amino acids, and phosphate. Musculoskeletal abnormalities such as joint hypermobility, dislocated hips, and fractures may develop as consequences of renal tubular acidosis and hypophosphatemia. Cataract is the only significant manifestation in carriers and is detected by slit-lamp examination.
    Defects in OCRL are the cause of Dent disease type 2 (DD2) [MIM:300555]. DD2 is a renal disease belonging to the 'Dent disease complex', a group of disorders characterized by proximal renal tubular defect, hypercalciuria, nephrocalcinosis, and renal insufficiency. The spectrum of phenotypic features is remarkably similar in the various disorders, except for differences in the severity of bone deformities and renal impairment. Characteristic abnormalities include low-molecular-weight proteinuria and other features of Fanconi syndrome, such as glycosuria, aminoaciduria, and phosphaturia, but typically do not include proximal renal tubular acidosis. Progressive renal failure is common, as are nephrocalcinosis and kidney stones.
  • 序列相似性Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type II family.
    Contains 1 Rho-GAP domain.
  • 细胞定位Endosome. Also found on macropinosomes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 3.1.3.36 antibody
    • Inositol polyphosphate 5 phosphatase OCRL 1 antibody
    • Inositol polyphosphate 5 phosphatase OCRL1 antibody
    • Inositol polyphosphate 5-phosphatase OCRL-1 antibody
    • INPP5F antibody
    • LOCR antibody
    • Lowe oculocerebrorenal syndrome protein antibody
    • NPHL2 antibody
    • OCRL 1 antibody
    • OCRL antibody
    • OCRL_HUMAN antibody
    • OCRL1 antibody
    • Oculocerebrorenal syndrome of Lowe antibody
    • Phosphatidylinositol polyphosphate 5 phosphatase antibody
    see all

Anti-INPP5F antibody 图像

  • All lanes : Anti-INPP5F antibody (ab125917) at 1/500 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.
  • ab125917, at 1/500, staining INPP5F in Human A431 cells (methanol-fixed) by immunofluorescence (panel 1) and co-stained with Hoechst 33342 (panel 2).
  • ab125917, at 1/250, staining INPP5F in Human Breast ca tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

Anti-INPP5F antibody (ab125917)参考文献

ab125917 has not yet been referenced specifically in any publications.

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