重组Anti-INPP4B抗体[EPR3108Y] (ab81269)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3108Y] to INPP4B
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-INPP4B抗体[EPR3108Y]
参阅全部 INPP4B 一抗 -
描述
兔单克隆抗体[EPR3108Y] to INPP4B -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human INPP4B aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: O15327 -
阳性对照
- WB: MCF7 and A-204 whole cell lysate; Human skeletal muscle lysate Flow Cyt (intra): MCF7 cells; IHC:human lung carcinoma tissue, human breast carcinoma tissue; ICC: MCF7 cells;
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3108Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Unpurified version to be used at 1/100 dilution. |
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ICC/IF |
1/50.
|
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WB |
1/1000 - 1/5000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa).
|
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IHC-P |
1/50.
Perform heat mediated antigen retrieval by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. |
说明 |
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Unpurified version to be used at 1/100 dilution. |
ICC/IF
1/50. |
WB
1/1000 - 1/5000. Detects a band of approximately 107 kDa (predicted molecular weight: 107 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval by microwaving the slides in Tris-EDTA buffer pH 9 at 650 W for 30 minutes. |
靶标
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功能
Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate. -
组织特异性
Widely expressed with highest levels occurring in the skeletal muscle and heart. -
通路
Signal transduction; phosphatidylinositol signaling pathway. -
序列相似性
Belongs to the inositol-3,4-bisphosphate 4-phosphatase family.
Contains 1 C2 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 8821 Human
- Omim: 607494 Human
- SwissProt: O15327 Human
- Unigene: 658245 Human
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别名
- Inositol polyphosphate 4 phosphatase, type II antibody
- 4-bisphosphate 4-phosphatase antibody
- Inositol polyphosphate 4 phosphatase type II 105kDa antibody
see all
图片
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All lanes : Anti-INPP4B antibody [EPR3108Y] (ab81269) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : A-204 (Human muscle rhabdomyosarcoma) whole cell lysate
Lane 4 : PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 107 kDa
Observed band size: 107 kDaINPP4B is lowly expressed in PANC-1 (PMID: 29952716)
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Immunocytochemistry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling INPP4B with Purified ab81269 at 1:50 dilution (2.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling INPP4B with Purified ab81269 at 1:50 dilution (2.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling INPP4B with Purified ab81269 at 1:50 dilution (2.36 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
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Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling INPP4B with purified ab81269 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab81269 被引用在 12 文献中.
- Ahmed AA et al. NanoString Digital Molecular Profiling of Protein and microRNA in Rhabdomyosarcoma. Cancers (Basel) 14:N/A (2022). PubMed: 35158790
- Wu Y et al. Expression and functional characterization of INPP4B in gallbladder cancer patients and gallbladder cancer cells. BMC Cancer 21:433 (2021). PubMed: 33879096
- Liu H et al. The INPP4B Tumor Suppressor Modulates EGFR Trafficking and Promotes Triple-Negative Breast Cancer. Cancer Discov 10:1226-1239 (2020). PubMed: 32513774
- Chaurasiya S et al. Estrogen receptor ß regulates AKT activity through up-regulation of INPP4B and inhibits migration of prostate cancer cell line PC-3. Proc Natl Acad Sci U S A 117:26347-26355 (2020). PubMed: 33020300
- Wang H et al. Feedback Activation of SGK3 and AKT Contributes to Rapamycin Resistance by Reactivating mTORC1/4EBP1 Axis via TSC2 in Breast Cancer. Int J Biol Sci 15:929-941 (2019). PubMed: 31182914