使用敲除细胞株进行验证RabMAb

Anti-INPP4A抗体[EP3425(2)] (ab109622)

概述

  • 产品名称Anti-INPP4A抗体[EP3425(2)]
    参阅全部 INPP4A 一抗
  • 描述
    兔单克隆抗体[EP3425(2)] to INPP4A
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human INPP4A.

  • 阳性对照
    • Fetal brain and Jurkat cell lysates. Human brain tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab109622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 104 kDa (predicted molecular weight: 110 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-bisphosphate.
    • 组织特异性Isoform 1 is expressed in the platelets, MEG-01 megakaryocytes and Jurkat T-cells. Isoform 2 is expressed in the brain.
    • 通路Signal transduction; phosphatidylinositol signaling pathway.
    • 序列相似性Belongs to the inositol-3,4-bisphosphate 4-phosphatase family.
      Contains 1 C2 domain.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 107kDa antibody
      • 4-bisphosphate 4-phosphatase antibody
      • 9630012D15 antibody
      • D130048C09Rik antibody
      • Inositol polyphosphate 4 phosphatase type I 107kDa antibody
      • Inositol polyphosphate 4 phosphatase type I antibody
      • Inositol polyphosphate 4-phosphatase type I antibody
      • INP4A_HUMAN antibody
      • INPP4 antibody
      • Inpp4a antibody
      • OTTHUMP00000203138 antibody
      • OTTHUMP00000203139 antibody
      • OTTHUMP00000203141 antibody
      • OTTHUMP00000225217 antibody
      • R74740 antibody
      • TVAS1 antibody
      • Type I inositol 3 4 bisphosphate 4 phosphatase antibody
      • Type I inositol 3,4 bisphosphate 4 phosphatase antibody
      • Type I inositol-3 antibody
      see all

    Anti-INPP4A antibody [EP3425(2)] 图像



    • Predicted band size : 110 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: INPP4A knockout HAP1 cell lysate (20 µg)
      Lane 3: Jurkat cell lysate (20 µg)
      Lane 4: Human fœtal brain tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109622 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109622 was shown to specifically react with INPP4A when INPP4A knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP4A knockout samples were subjected to SDS-PAGE.  Ab109622 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 110 kDa
      Observed band size : 104 kDa (why is the actual band size different from the predicted?)
    • ab109622 at 1/100 dilution staining INPP4A in paraffin-embedded Human brain tissue by Immunohistochemistry.

    Anti-INPP4A antibody [EP3425(2)] (ab109622)参考文献

    ab109622 has not yet been referenced specifically in any publications.

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