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Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab15323 as a replacement.
Our Abpromise guarantee covers the use of ab49999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/1000. Predicted molecular weight: 131 kDa.|
Immunocytochemistry/ Immunofluorescence analysis of IFN-γ/LPS-activated RAW-264.7 cells labeling iNOS with ab49999 at 1/2000 dilution. Cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton™ X-100. The antibody was developed using Goat Anti-Mouse IgG, Cy3-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.