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Our Abpromise guarantee covers the use of ab49999 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/1000. Predicted molecular weight: 131 kDa.|
Immunocytochemistry/ Immunofluorescence analysis of IFN-γ/LPS-activated RAW-264.7 cells labeling iNOS with ab49999 at 1/2000 dilution. Cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton™ X-100. The antibody was developed using Goat Anti-Mouse IgG, Cy3-conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.