Customer reviews and FAQs

概述

  • 产品名称
    Anti-INO80E抗体
  • 描述
    兔多克隆抗体to INO80E
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: LPRKLKMAVG PPDCPVGGPL TFPGRGSGAG VGTTLTPLPP PKMPPPTILS TVPRQMFSDA GSGDDALDGD DD, corresponding to internal sequence amino acids 166-237 of Human INO80E.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Human colon tissue; RT 4, U 251 MG and Human Tonsil lysates; U 251 MG cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab122837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Ccdc85 antibody
      • CCDC95 antibody
      • Coiled-coil domain-containing protein 95 antibody
      • FLJ90652 antibody
      • IN80E_HUMAN antibody
      • INO80 complex subunit E antibody
      • INO80E antibody
      see all

    Anti-INO80E antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80E antibody (ab122837)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80E antibody (ab122837)
      ab122837 at 1/1000 dilution staining INO80E in Paraffin-embedded Human colon tissue by Immunohistochemistry.
    • Western blot - Anti-INO80E antibody (ab122837)
      Western blot - Anti-INO80E antibody (ab122837)
      All lanes : Anti-INO80E antibody (ab122837) at 1/250 dilution

      Lane 1 : RT 4 lysate
      Lane 2 : U 251 MG lysate
      Lane 3 : Human Plasma lysate
      Lane 4 : Human Liver lysate
      Lane 5 : Human Tonsil lysate

      Developed using the ECL technique
    • Immunocytochemistry/ Immunofluorescence - Anti-INO80E antibody (ab122837)
      Immunocytochemistry/ Immunofluorescence - Anti-INO80E antibody (ab122837)
      ab122837 at 4 µg/ml staining INO80E in PFA/Triton X-100 fixed/permeabilized U 251 cells by Immunofluorescence (green).

    Anti-INO80E antibody (ab122837)参考文献

    ab122837 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab122837？请让我们知道，以便我们可以引用本数据表中的参考文章。

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