功能DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle.
组织特异性According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.
序列相似性Belongs to the SNF2/RAD54 helicase family. Contains 1 DBINO domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
结构域The DBINO region is involved in binding to DNA.
细胞定位Nucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner.
Detection of INO80 in Immunoprecipitates of Hela whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118787 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
Anti-INO80 antibody (ab118787)参考文献
This product has been referenced in:
Seo J et al. Genome-wide reorganization of histone H2AX toward particular fragile sites on cell activation. Nucleic Acids ResN/A:N/A (2013).
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