• 产品名称Anti-INO80抗体
    参阅全部 INO80 一抗
  • 描述
    兔多克隆抗体to INO80
  • 经测试应用适用于: ICC/IF, WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog, Pig, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1500 to the C-terminus of Human INO80.

  • 阳性对照
    • This antibody gave a positive signal in Human Brain tissue lysate and HeLa whole cell lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab105451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).
IHC-Fr Use at an assay dependent concentration.


  • 功能DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle.
  • 组织特异性According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas.
  • 序列相似性Belongs to the SNF2/RAD54 helicase family.
    Contains 1 DBINO domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 结构域The DBINO region is involved in binding to DNA.
  • 细胞定位Nucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA helicase INO80 antibody
    • hINO80 antibody
    • homolog of yeast INO80 antibody
    • INO80 antibody
    • INO80 complex subunit A antibody
    • INO80 homolog antibody
    • INO80_HUMAN antibody
    • INO80A antibody
    • INOC1 antibody
    • KIAA1259 antibody
    • Putative DNA helicase INO80 complex homolog 1 antibody
    see all

Anti-INO80 antibody 图像

  • All lanes : Anti-INO80 antibody (ab105451) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 177 kDa
    Observed band size : 177 kDa
    Additional bands at : 32 kDa,64 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab105451 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105451, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2, MCF7 cells at 1µg/ml.

Anti-INO80 antibody (ab105451)参考文献

ab105451 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Based on the sequence homology with the immunogen used to generate this antibody there is low likelihood of reactivity with S.cerevisiae.

I hope this information is helpful to you. Please do not hesitate to contact...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Mouse embryo OCT section e16.5)
Specification Mouse embryo OCT section e16.5
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Mr. Andrew McKay

Verified customer

提交于 Oct 25 2012

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product qu...

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I am very pleased to hear you would like to accept our offer and test ab105451 in mouse. This code will give you a discount off your next order before the expiration date. To redeem this offer, please submit an Abreview for mouse and include...

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Thank you very much for your interest in ab105451. We have tested and would guarantee this antibody to work in WB and ICC/IF using human samples.

Would your KO be in mouse? We don't have sample sizes of our products because we carry so many a...

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