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Our Abpromise guarantee covers the use of ab73904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Predicted molecular weight: 38 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
ab73904 staining Inhibin beta C chain in human embryonal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with Bouin's solution and blocked; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/1000) for 16 hours at 4°C. An undiluted HRP-conjugated anti-mouse IgG polyclonal was used as the secondary antibody.