Anti-Inhibin alpha抗体[R1] (ab14087)

概述

  • 产品名称Anti-Inhibin alpha抗体[R1]
    参阅全部 Inhibin alpha 一抗
  • 描述
    小鼠单克隆抗体[R1] to Inhibin alpha
  • 经测试应用适用于: WB, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Synthetic peptide corresponding to Human Inhibin alpha aa 233-264. Note that this peptide corresponds to the N-terminus of the mature region of the human inhibin alpha subunit.
    Sequence:

    STPLMSWPWSPSALRLLQRPPEEPAAHANCHR


    Database link: P05111

  • 阳性对照
    • human ovary, testis, placenta
  • 常规说明


    Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.08% Sodium Azide
    Constituents: Tissue Culture Supernatant
  • 纯度Tissue culture supernatant
  • Primary antibody说明Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumours, placental and gestational trophoblastic lesions and sex cord stromal tumours.
  • 克隆单克隆
  • 克隆编号R1
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab14087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/200. Predicted molecular weight: 32 kDa.
IHC-Fr 1/1 - 1/50.
IHC-P 1/1 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Neat - 1/1/50. Sodium citrate pH 6.0 is recommended for antigen retrieval.

靶标

  • 功能Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • 组织特异性Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.
  • 序列相似性Belongs to the TGF-beta family.
  • 翻译后修饰Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A inhibin subunit antibody
    • A inhibin subunit precursor antibody
    • AW55578 antibody
    • IHA antibody
    • inhA antibody
    • INHA_HUMAN antibody
    • Inhibin alpha chain antibody
    • Inhibin alpha chain precursor antibody
    • Inhibin alpha subunit antibody
    see all

Anti-Inhibin alpha antibody [R1] 图像

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of paraffin embedded human ovary tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue (showing sertoli cells, leydig cells and spermatogonia) labeling inhibin alpha with ab14087.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human testis tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Blocking with 0.3% H2O2 and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling inhibin alpha with ab14087. Following antigen retrieval in Tris/EDTA pH 9.0. Blocking with peroxidase blocking reagent and 10% normal goat serum. Visualization was with DAB substrate and counter-staining with haematoxylin.

Anti-Inhibin alpha antibody [R1] (ab14087)参考文献

ab14087 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Ovary (abnormal))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween 20, pH 6.0
Permeabilization No
Specification Ovary (abnormal)
Blocking step ab127055 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 21°C
Fixative Formaldehyde
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提交于 Aug 17 2016

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