Anti-Inhibin alpha抗体(ab81234)


  • 产品名称Anti-Inhibin alpha抗体
    参阅全部 Inhibin alpha 一抗
  • 描述
    兔多克隆抗体to Inhibin alpha
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Goat, Horse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 109-158 (


    ) of Human Inhibin alpha (NP_002182)

  • 阳性对照
    • Human fetal liver lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MCF-7



Our Abpromise guarantee covers the use of ab81234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

ICC/IF Use a concentration of 10 µg/ml.


  • 功能Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • 组织特异性Originally found in ovary (granulosa cells) and testis (Sertoli cells), but widely distributed in many tissues including brain and placenta. In adrenal cortex expression is limited to the zona reticularis and the innermost zona fasciculata in the normal gland, extending centripetally into the zona fasciculata in hyperplasia. Also found in adrenocortical tumors. Also expressed in prostate epithelium of benign prostatic hyperplasia, in regions of basal cell hyperplasia and in nonmalignant regions of high grade prostate cancer. Only circulating inhibin B is found in male, whereas circulating inhibins A and B are found in female.
  • 序列相似性Belongs to the TGF-beta family.
  • 翻译后修饰Proteolytic processing yields a number of bioactive forms. The 20/23 kDa forms consist solely of the mature alpha chain, the 26/29 kDa forms consist of the most N-terminal propeptide linked through a disulfide bond to the mature alpha chain, the 50/53 kDa forms encompass the entire proprotein. Each type can be furthermore either mono- or diglycosylated, causing the mass difference.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A inhibin subunit antibody
    • A inhibin subunit precursor antibody
    • IHA antibody
    • inhA antibody
    • INHA_HUMAN antibody
    • Inhibin alpha chain antibody
    • Inhibin alpha chain precursor antibody
    • Inhibin alpha subunit antibody
    see all

Anti-Inhibin alpha antibody 图像

  • ICC/IF image of ab81234 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81234 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-Inhibin alpha antibody (ab81234) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal liver lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%

Anti-Inhibin alpha antibody (ab81234)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Odyssey Blocking Buffer as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Sample Human Cell (COV434 cell line)
Specification COV434 cell line
Permeabilization Yes - Ethanol
Fixative Formaldehyde


Verified customer

提交于 May 30 2014

Application Western blot
Loading amount 60 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12)
Sample Human Cell lysate - whole cell (COV434 cell pellet)
Specification COV434 cell pellet
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C


Verified customer

提交于 Mar 12 2014

Thank you for your enquiry and your interest in our products. Unit size, price, availability, tested applications and species can be found on the on-line datasheets:

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