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ING4 is moderately similar to ING1 and may act in chromatin-mediated transcriptional regulation. ING4 contains a PHD-finger.
Our Abpromise guarantee covers the use of ab3714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).|
A 4-20% Tris-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rabbit anti Goat IgG [H&L] for 45 min at room temperature. LICOR's Odyssey® Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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