Anti-ING1抗体(ab22664)

概述

  • 产品名称Anti-ING1抗体
    参阅全部 ING1 一抗
  • 描述
    兔单克隆抗体to ING1
  • 特异性This antibodt detects p33 ING1 but does not cross react with p24 ING or p47 ING.
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse ING1 aa 2-14 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The immunogen is slightly different from the currently published mouse sequence, which is LSPANGEQIHLVN.
    Sequence:

    LSPANEQLHLVN(C)

  • 阳性对照
    • Mouse brain tissue extract.

性能

应用

Our Abpromise guarantee covers the use of ab22664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ELISA Use a concentration of 1 - 2 µg/ml.

靶标

  • 功能Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene.
  • 组织特异性Isoform 2 was expressed in all normal tissues and cells examined, as well as in all breast cancer and melanoma cell lines examined. Isoform 3 was expressed in testis, liver, and kidney, weakly expressed in colon and brain and not expressed in breast and cultured melanocytes. Isoform 4 was highly expressed in testis and weakly expressed in brain, but not expressed in breast, colon, kidney, melanocytes, breast cancer or melanoma cell lines.
  • 疾病相关Defects in ING1 are a cause of head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck.
  • 序列相似性Belongs to the ING family.
    Contains 1 PHD-type zinc finger.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2610028J21Rik antibody
    • AA407184 antibody
    • AI875420 antibody
    • Growth inhibitor ING 1 antibody
    • Growth inhibitor ING1 antibody
    • Growth inhibitory protein ING 1 antibody
    • Growth inhibitory protein ING1 antibody
    • Homo sapiens growth inhibitor p33ING1 (ING1) mRNA, complete cds antibody
    • ING 1 antibody
    • Ing1 antibody
    • ING1_HUMAN antibody
    • Inhibitor of growth 1 antibody
    • Inhibitor of growth family member 1 antibody
    • Inhibitor of growth protein 1 antibody
    • mING1h antibody
    • OTTHUMP00000018703 antibody
    • OTTHUMP00000018704 antibody
    • OTTHUMP00000018705 antibody
    • OTTHUMP00000018706 antibody
    • p24ING1c antibody
    • p33 antibody
    • p33 ING1 antibody
    • p33ING1 antibody
    • p33ING1b antibody
    • p33ING1c antibody
    • p37Ing1b antibody
    • p47 antibody
    • p47ING1a antibody
    • Tumor suppressor ING 1 antibody
    • Tumor suppressor ING1 antibody
    see all

Anti-ING1 antibody 图像

  • All lanes : Anti-ING1 antibody (ab22664) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 26 kDa,40 kDa. We are unsure as to the identity of these extra bands.

Anti-ING1 antibody (ab22664)参考文献

ab22664 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply. This antibody has been used successfully with human and mouse samples. We do not have any sample sizes, but we guarantee that the antibody works in the tested species and applications listed on the datasheet. I am not...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"