The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Can also be used for detection and serotyping of influenza virus type A.
Inhibit viral reproduction after fatty acid acylation.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
1)Cell monolayer was grown on a glass and infected with the virus for 2 days at 37 oC.
2)Glass was dried and the cells were fixed with cold acetone at 4 oC for 10 min.
3)Ab8261 was introduced in concentrations from 1 to 100 µg/ml in PBS and incubated for 30 min in RT.
4)Glass was washed with PBS several times and incubated with goat anti-mouse IgG labeled with FITC.
The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1.
"NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
Host cell nucleus
Common flu NP antibody
Influenza A virus NP antibody
Nucleocapsid protein antibody
Protein N antibody
Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
Anti-Influenza A Virus Nucleoprotein antibody [IV F8] (ab8261)参考文献
This product has been referenced in:
Yan Y et al. Human nasal epithelial cells derived from multiple subjects exhibit differential responses to H3N2 influenza virus infection in vitro. J Allergy Clin Immunol138:276-281.e15 (2016).
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