抗甲型流感Virus Nucleoprotein抗体[9G8] (ab43821)
Key features and details
- Mouse monoclonal [9G8] to Influenza A Virus Nucleoprotein
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Influenza A
- Isotype: IgG2a
概述
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产品名称
抗甲型流感Virus Nucleoprotein抗体[9G8]
参阅全部 Influenza A Virus Nucleoprotein 一抗 -
描述
小鼠单克隆抗体[9G8] to甲型流感Virus Nucleoprotein -
宿主
Mouse -
经测试应用
适用于: ELISA, WB, ICC/IFmore details -
种属反应性
与反应: Influenza A -
免疫原
Recombinant full length protein corresponding to Influenza A Virus Nucleoprotein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
9G8 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab43821于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
1/2000 - 1/10000.
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WB |
1/200 - 1/1000.
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|
ICC/IF |
1/100 - 1/500.
On infected cells |
说明 |
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ELISA
1/2000 - 1/10000. |
WB
1/200 - 1/1000. |
ICC/IF
1/100 - 1/500. On infected cells |
靶标
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相关性
The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002). -
细胞定位
Host cell nucleus -
数据库链接
- Entrez Gene: 956531 Influenza A
- SwissProt: P03466 Influenza A
- SwissProt: P69291 Influenza A
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别名
- Common flu NP antibody
- Influenza A virus NP antibody
- NP antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab43821 被引用在 6 文献中.
- Ganti K et al. Rab11a mediates cell-cell spread and reassortment of influenza A virus genomes via tunneling nanotubes. PLoS Pathog 17:e1009321 (2021). PubMed: 34473799
- Harbig A et al. Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways. J Biol Chem 295:11388-11407 (2020). PubMed: 32303635
- Phipps KL et al. Collective interactions augment influenza A virus replication in a host-dependent manner. Nat Microbiol 5:1158-1169 (2020). PubMed: 32632248
- Seo YJ et al. Sphingosine kinase 1 serves as a pro-viral factor by regulating viral RNA synthesis and nuclear export of viral ribonucleoprotein complex upon influenza virus infection. PLoS One 8:e75005 (2013). WB . PubMed: 24137500
- Dimmock NJ et al. Comparison of the protection of ferrets against pandemic 2009 influenza A virus (H1N1) by 244 DI influenza virus and oseltamivir. Antiviral Res 96:376-85 (2012). PubMed: 23041142
- Killingley B et al. Virus shedding and environmental deposition of novel A (H1N1) pandemic influenza virus: interim findings. Health Technol Assess 14:237-354 (2010). PubMed: 20923613