Anti-Influenza A Virus M2 Protein抗体[14C2] (ab5416)

概述

  • 产品名称
    Anti-Influenza A Virus M2 Protein抗体[14C2]
    参阅全部 Influenza A Virus M2 Protein 一抗
  • 描述
    小鼠单克隆抗体[14C2] to Influenza A Virus M2 Protein
  • 经测试应用
    适用于: Inhibition Assay, Flow Cyt, ICC/IF, ICC, IP, WBmore details
  • 免疫原

    Tissue, cells or virus corresponding to Influenza A Virus M2 Protein. Full length M2 protein from A/WSN/33-infected CV1 cell lysate.

  • 表位
    Detects the N-terminal of the Influenza A Virus M2 Protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度
    Protein G purified
  • Primary antibody说明
    Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
  • 克隆
    单克隆
  • 克隆编号
    14C2
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Inhibition Assay Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa.

靶标

  • 相关性
    The Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
  • 细胞定位
    Virion membrane. Apical cell membrane; Single-pass type III membrane protein.
  • 别名
    • Influenza A virus matrix protein M2 antibody
    • M antibody
    • M2 antibody
    • Matrix protein 2 antibody
    • Membrane ion channel M2 antibody
    • Membrane protein M2 antibody
    • Proton channel protein M2 antibody
    see all

图片

  • Immunofluorescence staining of infected MDCK cells using ab5416.

文献

This product has been referenced in:
  • Zhu P  et al. Host Cellular Protein TRAPPC6A? Interacts with Influenza A Virus M2 Protein and Regulates Viral Propagation by Modulating M2 Trafficking. J Virol 91:N/A (2017). Read more (PubMed: 27795429) »
  • Turnbull ML  et al. Role of the B Allele of Influenza A Virus Segment 8 in Setting Mammalian Host Range and Pathogenicity. J Virol 90:9263-84 (2016). Read more (PubMed: 27489273) »

See all 26 Publications for this product

客户评价及客户问答

Thank you very much for your patience.

Finally, I have a price quote and custom made confirmation from laboratory. We are happy to provide this product at lower cost than ab5416. The price would be £194 per vial or £582 for 3 vial...

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Thank you for your email.

I have sent a request to laboratory for quote; I will send you the details soon.

Many thanks for having patience!

Thank you for your email.

I just spoken to my colleague in laboratory; they have suggested that in order to justify the production cost for making it BSA and azide free at least 3 vials should be ordered. Could you let me know if you will buy ...

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Thank you for contacting us.

We can provide this product, BSA and azide free as a special request. Could you let me know how many vials you would like to buy?

Looking forward to hearing from you soon.

Thank you for contacting us.

Our Influenza A M2 Monoclonal Antibody (14C2) - product #ab5416 was shown to detect influenza A virus M2 strain A/PR/8/34 in the following reference:



Zebedee SL, Lamb RA"Influenza A vi...

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Thank you foryour reply and forkindly providing the requested information.

I have investigated this with our source for the antibody and reviewed the details. It is worth consideringthat the Nandrop will be unable to distinguish between IgG ...

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Thank you for contacting Abcam regarding this,
I have had the chance to speak with the lab about your question. We have not tested whether this antibody can be used as a neutralizing antibody. The authors in the following paper used this antibody i...

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I would suggest to prepare buffers that are recommended in the following Western Blotting protocol : http://www.abcam.com/index.html?pageconfig=resource&rid=13045 .

Thank you very much for your inquiry. I have contacted the laboratory for information on the ab5416. However the laboratory has not tested this antibody neither on your strain. I did a sequence alignement of the immunogen and your sequence. Please see ...

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Thank you for your enquiry. This product was created against whole M2 protein purified from A/WSN/33-infected CV1 cell lysate. Unfortunately, the exact epitope for this polyclonal antibody is not known at this time. The antibody was not tested aga...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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