The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Influenza A virus is a major public health threat, killing more
than 30,000 people per year in the USA. The virus has one
of sixteen possible hemagglutinin (HA) surface proteins and
one of nine possible neuraminidase (NA) surface proteins. In
early 2009, a novel H1N1 swine-origin influenza (S-OIV) A
virus was identified in specimens obtained from patients in
Mexico and the United States. The genetic make-up of this
swine flu virus is unlike any other: it is an H1N1 strain that
combines a triple assortment first identified in 1998 including
human, swine, and avian influenza with two new pig H3N2
virus genes from Eurasia, themselves of recent human origin. One of the less studied proteins encoded by, but not
incorporated in, the influenza virus is the nonstructural protein
(NS) 1. NS1 counters cellular antiviral activities and acts as a
virulence factor. It can bind to double-stranded RNA and
sequester it from 2’-5’ OAS, preventing the activation of the
RNAse L, which normally acts to degrade RNA and prevent
virus replication. NS1 also binds to and inhibits the antiviral
protein kinase PKR.
host cell cytoplasm and nucleus
Nonstructural protein 1 antibody
has not yet been referenced specifically in any publications.