RabMAb

Anti-Indoleamine 2, 3-dioxygenase抗体[EPR1230Y] (ab76157)

概述

  • 产品名称Anti-Indoleamine 2, 3-dioxygenase抗体[EPR1230Y]
    参阅全部 Indoleamine 2, 3-dioxygenase 一抗
  • 描述
    兔单克隆抗体[EPR1230Y] to Indoleamine 2, 3-dioxygenase
  • 经测试应用适用于: ICC/IF, WBmore details
    不适用于: Flow Cyt,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues within human Indoleamine 2, 3-dioxygenase

  • 阳性对照
    • WB: HeLa cell lysate treated with IFN gamma. ICC/IF: HepG2 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab76157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/2500 - 1/5000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
  • 应用说明Is unsuitable for Flow Cyt,IHC-P or IP.
  • 靶标

    • 功能Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
    • 通路Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
    • 序列相似性Belongs to the indoleamine 2,3-dioxygenase family.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 3-dioxygenase antibody
      • I23O1_HUMAN antibody
      • IDO 1 antibody
      • IDO antibody
      • IDO-1 antibody
      • IDO1 antibody
      • INDO antibody
      • indolamine 2,3 dioxygenase antibody
      • Indole 2 3 dioxygenase antibody
      • indoleamine 2 3 dioxygenase 1 antibody
      • indoleamine 2 3 dioxygenase antibody
      • Indoleamine 2,3-dioxygenase 1 antibody
      • Indoleamine pyrrole 2 3 dioxygenase antibody
      • Indoleamine-pyrrole 2 antibody
      see all

    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] 图像

    • ab76157 stained HepG2 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76157 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/5000 dilution + HeLa cell lysate treated with IFN gamma at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa

    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)参考文献

    This product has been referenced in:
    • Yamazaki T  et al. Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in human epithelial HeLa cells treated with interferon-?. J Infect Chemother 20:460-4 (2014). Human . Read more (PubMed: 24855914) »
    • De Ravin SS  et al. Tryptophan/kynurenine metabolism in human leukocytes is independent of superoxide and is fully maintained in chronic granulomatous disease. Blood 116:1755-60 (2010). Read more (PubMed: 20511543) »

    See all 2 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Loading amount 60 µg
    Gel Running Conditions Non-reduced Denaturing (gel 10 %)
    Sample Dog Cell lysate - whole cell (adipose tissue derived mesencymal stem cell)
    Specification adipose tissue derived mesencymal stem cell
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Username

    Dr. SeungHoon Lee

    Verified customer

    提交于 Sep 12 2013

    Following up my previous message, I received confirmation that, for the IDO antibody ab76157,  no testing has been done on rat samples. I can send this as a replacement but if you would rather have a credit or refund for ab106134, I can issue that inst...

    Read More

    The producer of this clone has provided the following information about the immunogen for ab76157: "Sequence homology of the immunogen with rat IDO is 86%. Species cross-reactivity data seems to be missing, so I will update you soon." It is not c...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"