重组人TrkC蛋白(Fc Chimera) (ab85599)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Suitable for: Functional Studies, SDS-PAGE
描述
-
产品名称
重组人TrkC蛋白(Fc Chimera)
参阅全部 TrkC 蛋白酶 -
纯度
> 95 % SDS-PAGE. -
表达系统
HEK 293 cells -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
Theoretical Sequence: CPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNANINITDISRN ITSIHIENWRSLHTLNAVDMELYTGLQKLTIKNSGLRSIQPRAFAKNPHL RYINLSSNRLTTLSWQLFQTLSLRELQLEQNFFNCSCDIRWMQLWQEQGE AKLNSQNLYCINADGSQLPLFRMNISQCDLPEISVSHVNLTVREGDNAVI TCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNG FTLTCIAENVVGMSNASVALTVYYPPRVVSLEEPELRLEHCIEFVVRGNP PPTLHWLHNGQPLRESKIIHVEYYQEGEISEGCLLFNKPTHYNNGNYTLI AKNPLGTANQTINGHFLKEPFPESTDNFILFDEVSPTPPITVTHKPEEDG SSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSR DELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFF LYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK -
氨基酸
1 to 428 -
额外的序列信息
Fused with the Fc region of Human IgG1 at the C-terminus.
-
技术指标
Our Abpromise guarantee covers the use of ab85599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
SDS-PAGE
-
形式
Lyophilized -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Store at +4°C.
Constituents: PBS, 1% Human serum albumin, 10% Trehalose
-
复溶Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
常规信息
-
别名
- EC 2.7.10.1
- ETS related protein neurotrophic receptor tyrosine kinase fusion
- ETS related protein neurotrophic receptor tyrosine kinase fusion protein
see all -
功能
Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties. -
组织特异性
Widely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 protein kinase domain. -
翻译后修饰
Ligand-mediated auto-phosphorylation. -
细胞定位
Membrane. - Information by UniProt
图片
-
1D SDS-PAGE of ab85599 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab85599
Lane 2: ab85599 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab85599 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
5 mg of protein was loaded per lane. Gel was stained with Deep Purple™.Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent tightening of bands after treatment with glycosidase cocktail suggests the presence of O-linked glycans.
-
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab85599 尚未被引用在任何文献中。