概述

  • 产品名称
    Anti-Importin4抗体
    参阅全部 Importin4 一抗
  • 描述
    兔多克隆抗体to Importin4
  • 经测试应用
    适用于: IP, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 350-400 (CPQLMPMLEE ALRSESPYQR KAGLLVLAVL SDGAGDHIRQ RLLPPLLQIV C) of Human Importin4 (NP_078934.3).

  • 阳性对照
    • HeLa whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab99270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa. This antibody has not performed satisfactorily when used for WB of Importin4 in crude preparations (e.g. whole cell lysate). ab99270 can be used for WB of enriched (e.g. immunoprecipitated) sources of Importin4.

靶标

  • 功能
    Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
  • 序列相似性
    Belongs to the importin beta family.
    Contains 6 HEAT repeats.
    Contains 1 importin N-terminal domain.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Imp4 antibody
    • Imp4b antibody
    • Importin 4 antibody
    • Importin 4b antibody
    • Importin-4 antibody
    • Importin-4b antibody
    • Ipo4 antibody
    • IPO4_HUMAN antibody
    • Ran binding protein 4 antibody
    • Ran-binding protein 4 antibody
    • RanBP4 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Importin4 with ab99270 at 1/5000 (0.2µg/mg). Detection: DAB.
  • Detection of Importin4 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99270 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 5 seconds

文献

ab99270 has not yet been referenced specifically in any publications.

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