RabMAb

Anti-Importin 9抗体[EP1353Y] (ab52605)

概述

  • 产品名称Anti-Importin 9抗体[EP1353Y]
    参阅全部 Importin 9 一抗
  • 描述
    兔单克隆抗体[EP1353Y] to Importin 9
  • 经测试应用适用于: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide within Human Importin 9 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q96P70

  • 阳性对照
    • Fetal brain lysate; human lymphoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available: 
    Anti-Importin 9 antibody (Phycoerythrin) [EP1353Y] (ab208995)

性能

应用

Our Abpromise guarantee covers the use of ab52605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 115 kDa).
IP 1/20.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/250.

靶标

  • 功能Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
  • 序列相似性Belongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Imp9 antibody
    • Imp9a antibody
    • Imp9b antibody
    • Importin-9 antibody
    • Importin-9a antibody
    • Ipo9 antibody
    • IPO9_HUMAN antibody
    • Ran-binding protein 9 antibody
    • RanBP9 antibody
    see all

Anti-Importin 9 antibody [EP1353Y] 图像

  • Overlay histogram showing HeLa cells stained with ab52605 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52605, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-Importin 9 antibody [EP1353Y] (ab52605) at 1/1000 dilution + Fetal brain lysate at 10 µg

    Secondary
    HRP-labelled goat anti-rabbit. at 1/2000 dilution

    Predicted band size : 115 kDa
  • Immunohistochemical staining of paraffin-embedded human lymphoma using ab52605 at a dilution of 1/50.

Anti-Importin 9 antibody [EP1353Y] (ab52605)参考文献

This product has been referenced in:
  • You P  et al. Expression and subcellular distribution of imp13 are regulated in brain development. In Vitro Cell Dev Biol Anim 49:346-53 (2013). IHC-P ; Mouse . Read more (PubMed: 23605716) »
  • Ratnakumar K  et al. ATRX-mediated chromatin association of histone variant macroH2A1 regulates a-globin expression. Genes Dev 26:433-8 (2012). WB ; Human . Read more (PubMed: 22391447) »

See all 4 Publications for this product

Product Wall

I can confirm that ab52605 is against RanBP9 not RanBPM, my apologies for the fact that RanBPM was added as an alternative name on the datasheet, this has been corrected immediately. The detected band's molecular weight matches the predicted MW deta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"