概述

  • 产品名称Anti-Importin 7抗体
    参阅全部 Importin 7 一抗
  • 描述
    兔多克隆抗体to Importin 7
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
  • 免疫原

    A region within amino acids 50-100 of Human Importin 7 (NP_006382.1 ).

  • 阳性对照
    • HeLa whole cell lysate. 293T cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab99272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 120 kDa.
IP Use at 5-15 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
  • 序列相似性Belongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ14581 antibody
    • Imp 7 antibody
    • Imp7 antibody
    • Importin-7 antibody
    • Importin7 antibody
    • IPO 7 antibody
    • IPO7 antibody
    • IPO7_HUMAN antibody
    • MGC138673 antibody
    • Ran binding protein 7 antibody
    • Ran-binding protein 7 antibody
    • RANBP 7 antibody
    • RanBP7 antibody
    see all

Anti-Importin 7 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Importin 7 with ab99272 at 1/1000 (0.2µg/mg). Detection: DAB.
  • All lanes : Anti-Importin 7 antibody (ab99272) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 120 kDa


    Exposure time : 30 seconds
  • ab99272 at 0.4 µg/ml detecting Importin 7 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 30 second exposure.
    For immunoprecipitation, ab99272 was used at at 10 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99272
    Lane 2; IP using a rabbit anti-Importin 7 antibody which recognizes a downstream epitope.
    Lane 3; IP using control IgG.

Anti-Importin 7 antibody (ab99272)参考文献

ab99272 has not yet been referenced specifically in any publications.

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