概述

  • 产品名称Anti-Importin 7抗体
    参阅全部 Importin 7 一抗
  • 描述
    小鼠单克隆抗体to Importin 7
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Xenopus laevis, Zebrafish
  • 免疫原

    Recombinant fragment: DDEDNPVDEY QIFKAIFQTI QNRNPVWYQA LTHGLNEEQR KQLQDIATLA DQRRAAHESK MIEKHGGYKF SAPVVPSSFN FGGPAPGMN, corresponding to amino acids 950-1039 of Human Importin 7, with a 26 kDa tag, NP_006382.

  • 阳性对照
    • Recombinant protein (immunogen) and HeLa cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab88339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 115 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In vitro, mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
  • 序列相似性Belongs to the importin beta family.
    Contains 1 importin N-terminal domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ14581 antibody
    • Imp 7 antibody
    • Imp7 antibody
    • Importin-7 antibody
    • Importin7 antibody
    • IPO 7 antibody
    • IPO7 antibody
    • IPO7_HUMAN antibody
    • MGC138673 antibody
    • Ran binding protein 7 antibody
    • Ran-binding protein 7 antibody
    • RANBP 7 antibody
    • RanBP7 antibody
    see all

Anti-Importin 7 antibody 图像

  • Anti-Importin 7 antibody (ab88339) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 115 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Anti-Importin 7 antibody (ab88339) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 115 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)

Anti-Importin 7 antibody (ab88339)参考文献

ab88339 has not yet been referenced specifically in any publications.

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