概述

  • 产品名称Anti-IMPDH2抗体
    参阅全部 IMPDH2 一抗
  • 描述
    兔多克隆抗体to IMPDH2
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • 免疫原

    A synthetic peptide corresponding to a region of Human IMPDH2

  • 阳性对照
    • HepG2 cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab75790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-IMPDH2 antibody 图像

  • Anti-IMPDH2 antibody (ab75790) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • IHC image of ab75790 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75790, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab75790 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75790, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab75790 staining IMPDH2 in HEp-2 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody against IMPDH2 (red) and nuclei were stained with DAPI (blue).

Anti-IMPDH2 antibody (ab75790)参考文献

This product has been referenced in:
  • Fromm-Dornieden C  et al. Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. BMC Mol Biol 13:9 (2012). WB . Read more (PubMed: 22436005) »
  • Carcamo WC  et al. Induction of cytoplasmic rods and rings structures by inhibition of the CTP and GTP synthetic pathway in mammalian cells. PLoS One 6:e29690 (2011). ICC/IF ; Human . Read more (PubMed: 22220215) »

See all 2 Publications for this product

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