概述
- 产品名称Anti-IMPDH1抗体
参阅全部 IMPDH1 一抗 - 描述兔多克隆抗体to IMPDH1
- 经测试应用适用于: WB, IHC-Pmore details
- 种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- 免疫原
Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (RSKKGKLPIV NDCDELVAII ARTDLKKNRD YPLASKDSQK QLLCGAAVGT) of Human IMPDH1 (NP_899066).
- 阳性对照
- HeLa cell lysate.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab84957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use at an assay dependent concentration. |
靶标
- 功能Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
- 组织特异性IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
- 通路Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
- 疾病相关Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant.
- 序列相似性Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains. - Information by UniProt
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数据库链接
- Entrez Gene: 3614 Human
- Entrez Gene: 23917 Mouse
- Entrez Gene: 362329 Rat
- Omim: 146690 Human
- SwissProt: P20839 Human
- SwissProt: P50096 Mouse
- SwissProt: D3ZLZ7 Rat
- Unigene: 654401 Human
see all -
别名
- IMDH1_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 1 antibody
- IMP dehydrogenase 1 antibody
see all
Anti-IMPDH1 antibody 图像
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Western blot - IMPDH1 antibody (ab84957)Anti-IMPDH1 antibody (ab84957) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 55 kDa
Observed band size : 55 kDa
Additional bands at : 27 kDa,31 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody (ab84957)ab84957 staining IMPDH1 in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody (ab84957)ab84957 staining IMPDH1 in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Anti-IMPDH1 antibody (ab84957)参考文献
ab84957 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"