概述

  • 产品名称
    Anti-IMPDH1抗体
    参阅全部 IMPDH1 一抗
  • 描述
    兔多克隆抗体to IMPDH1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (RSKKGKLPIV NDCDELVAII ARTDLKKNRD YPLASKDSQK QLLCGAAVGT) of Human IMPDH1 (NP_899066).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • HeLa cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
  • 组织特异性
    IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
  • 通路
    Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
  • 疾病相关
    Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant.
  • 序列相似性
    Belongs to the IMPDH/GMPR family.
    Contains 2 CBS domains.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • IMDH1_HUMAN antibody
    • IMP (inosine monophosphate) dehydrogenase 1 antibody
    • IMP dehydrogenase 1 antibody
    • IMPD 1 antibody
    • IMPD antibody
    • IMPD1 antibody
    • IMPDH 1 antibody
    • IMPDH I antibody
    • IMPDH-I antibody
    • Impdh1 antibody
    • Inosine 5' monophosphate dehydrogenase 1 antibody
    • Inosine monophosphate dehydrogenase 1 antibody
    • Inosine-5''-monophosphate dehydrogenase 1 antibody
    • LCA11 antibody
    • RP10 antibody
    • sWSS2608 antibody
    see all

图片

  • Western blot - IMPDH1 antibody (ab84957)
    Western blot - IMPDH1 antibody (ab84957)
    Anti-IMPDH1 antibody (ab84957) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Additional bands at: 27 kDa, 31 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody (ab84957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody (ab84957)
    ab84957 staining IMPDH1 in human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody (ab84957)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH1 antibody (ab84957)
    ab84957 staining IMPDH1 in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

文献

This product has been referenced in:

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IMPDH1 antibody

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Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human cancer xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 9
Permeabilization
No
Specification
human cancer xenograft
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
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提交于 Jun 24 2015

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