This antibody gave a positive signal in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
Jurkat (Human T cell lymphoblast-like cell line)
A431 (Human epithelial carcinoma cell line)
NIH 3T3 (Mouse embryonic fibroblast cell line)
MEF1 (Mouse embryonic fibroblast cell line)
PC12 (Rat adrenal pheochromocytoma cell line)
This antibody gave a positive signal in the following tissue lysate:
The immunogen used for this product shares 86% homology with IMPDH2. Cross-reactivity with this protein has not been confirmed experimentally.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100 (please refer to abreview for further details).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant.
Belongs to the IMPDH/GMPR family. Contains 2 CBS domains.
All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 :Jurkat whole cell lysate (ab7899) Lane 3 :A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary All lanes : IRDye 680 Conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-IMPDH1 antibody (ab33039)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab33039 (1/100) staining IMPDH1 in HeLa cells in conjunction with a secondary goat anti-rabbit polyclonal antibody conjugated to Cy3 (1/200). Cells were counterstained with DAPI in order to expose the nucleus(red). Please refer to abreview for further details.