概述

  • 产品名称
  • 描述
    兔多克隆抗体to IMPDH1
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human IMPDH1.

    (Peptide available as ab33038.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) This antibody gave a positive signal in the following tissue lysate: Ovary (Mouse)
  • 常规说明

    The immunogen used for this product shares 86% homology with IMPDH2. Cross-reactivity with this protein has not been confirmed experimentally.

性能

应用

Our Abpromise guarantee covers the use of ab33039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
  • 应用说明
    ICC/IF: 1/100 (please refer to abreview for further details).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.
    • 组织特异性
      IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.
    • 通路
      Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.
    • 疾病相关
      Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10) [MIM:180105]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant.
    • 序列相似性
      Belongs to the IMPDH/GMPR family.
      Contains 2 CBS domains.
    • Information by UniProt
    • 数据库链接
    • 别名
      • IMDH1_HUMAN antibody
      • IMP (inosine monophosphate) dehydrogenase 1 antibody
      • IMP dehydrogenase 1 antibody
      • IMPD 1 antibody
      • IMPD antibody
      • IMPD1 antibody
      • IMPDH 1 antibody
      • IMPDH I antibody
      • IMPDH-I antibody
      • Impdh1 antibody
      • Inosine 5' monophosphate dehydrogenase 1 antibody
      • Inosine monophosphate dehydrogenase 1 antibody
      • Inosine-5''-monophosphate dehydrogenase 1 antibody
      • LCA11 antibody
      • RP10 antibody
      • sWSS2608 antibody
      see all

    Anti-IMPDH1 antibody 图像

    • All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat whole cell lysate (ab7899)
      Lane 3 : A431 whole cell lysate (ab7909)

      Lysates/proteins at 20 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat anti-rabbit IgG (H&L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 55 kDa
      Observed band size : 55 kDa
    • ab33039 (1/100) staining IMPDH1 in HeLa cells in conjunction with a secondary goat anti-rabbit polyclonal antibody conjugated to Cy3 (1/200). Cells were counterstained with DAPI in order to expose the nucleus(red). Please refer to abreview for further details.

      See Abreview

    • All lanes : Anti-IMPDH1 antibody (ab33039) at 1 µg/ml

      Lane 1 : NIH 3T3 whole cell lysate (ab7179)
      Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 3 : Ovary (Mouse) Tissue Lysate - normal tissue
      Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 55 kDa
      Observed band size : 55 kDa

    Anti-IMPDH1 antibody (ab33039)参考文献

    This product has been referenced in:
    • Kim HR  et al. A p53-inducible microRNA-34a downregulates Ras signaling by targeting IMPDH. Biochem Biophys Res Commun 418:682-8 (2012). WB ; Human . Read more (PubMed: 22301190) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lung epithelial)
    Loading amount
    10 µg
    Specification
    Lung epithelial
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 18 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Retina)
    Specification
    Retina
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 26 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Mar 19 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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