概述

  • 产品名称Anti-IMPACT抗体
    参阅全部 IMPACT 一抗
  • 描述
    兔多克隆抗体to IMPACT
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 270-320 (GILLGPDRFK HINNCARNIL VEKNYTNSPE ESSKALGKNK KVRKDKKRNE H) of Human IMPACT (NP_060909.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab95175 was affinity purified using an epitope specific to IMPACT immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab95175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 36 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Translational regulator that ensures constant high levels of translation under amino acid starvation. Acts by interacting with GCN1/GCN1L1, thereby preventing activation of GCN2 protein kinases (EIF2AK1 to 4) and subsequent down-regulation of protein synthesis.
  • 组织特异性Widely expressed. Expressed at high level in brain.
  • 序列相似性Belongs to the IMPACT family.
    Contains 1 RWD domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IMPACT antibody
    • Impact homolog (mouse) antibody
    • Impact homolog antibody
    • impact RWD domain protein antibody
    • IMPCT_HUMAN antibody
    • Imprinted and ancient gene protein homolog antibody
    • MGC33718 antibody
    • Protein IMPACT antibody
    • RWD domain containing 5 antibody
    • RWDD5 antibody
    see all

Anti-IMPACT antibody 图像

  • All lanes : Anti-IMPACT antibody (ab95175) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 36 kDa


    Exposure time : 30 seconds
  • Detection of IMPACT in immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab95175 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-IMPACT antibody (ab95175)参考文献

ab95175 has not yet been referenced specifically in any publications.

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