概述

  • 产品名称Anti-IMP3抗体
    参阅全部 IMP3 一抗
  • 描述
    小鼠单克隆抗体to IMP3
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human IMP3 (AAH65269, amino acids 1-579) with a proprietary tag.

  • 阳性对照
    • K562 cell lysate. Tagged recombinant Human IMP3 protein.

性能

应用

Our Abpromise guarantee covers the use of ab89521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.
  • 组织特异性Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.
  • 序列相似性Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • 结构域The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
  • 细胞定位Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancer/testis antigen 98 antibody
    • CT98 antibody
    • DKFZp686F1078 antibody
    • hKOC antibody
    • IF2B3_HUMAN antibody
    • IGF II mRNA binding protein 3 antibody
    • IGF-II mRNA-binding protein 3 antibody
    • IGF2 mRNA binding protein 3 antibody
    • IGF2 mRNA-binding protein 3 antibody
    • IGF2BP3 antibody
    • IMP 3 antibody
    • IMP-3 antibody
    • Insulin like growth factor 2 mRNA binding protein 3 antibody
    • Insulin-like growth factor 2 mRNA-binding protein 3 antibody
    • KH domain containing protein overexpressed in cancer antibody
    • KH domain-containing protein overexpressed in cancer antibody
    • KOC 1 antibody
    • KOC1 antibody
    • VICKZ 3 antibody
    • VICKZ family member 3 antibody
    • VICKZ3 antibody
    see all

Anti-IMP3 antibody 图像

  • Anti-IMP3 antibody (ab89521) at 1/500 dilution + Tagged recombinant Human IMP3 protein at 0.2 µg

    Predicted band size : 64 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • Anti-IMP3 antibody (ab89521) at 1/500 dilution + K562 cell lysate at 50 µg

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 36 kDa,46 kDa,48 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab89521 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89521, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IMP3 antibody (ab89521)参考文献

ab89521 has not yet been referenced specifically in any publications.

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