概述

  • 产品名称Anti-ILF3抗体
    参阅全部 ILF3 一抗
  • 描述
    兔多克隆抗体to ILF3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • K562 cell lysate and human lung tissue.

性能

应用

Our Abpromise guarantee covers the use of ab50832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 95 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能May facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. Can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. Can regulate protein arginine N-methyltransferase 1 activity. May regulate transcription of the IL2 gene during T-cell activation. Can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
  • 组织特异性Ubiquitous.
  • 序列相似性Contains 2 DRBM (double-stranded RNA-binding) domains.
    Contains 1 DZF domain.
  • 翻译后修饰Phosphorylated by RNA-dependent protein kinase (EIF2AK2).
    Methylated by protein arginine N-methyltransferase 1.
    Arg-609 is dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBTF antibody
    • Double stranded RNA binding protein 76 antibody
    • Double-stranded RNA-binding protein 76 antibody
    • DRBF antibody
    • DRBP76 antibody
    • ILF3 antibody
    • ILF3_HUMAN antibody
    • Interleukin enhancer binding factor 3 antibody
    • Interleukin enhancer-binding factor 3 antibody
    • M phase phosphoprotein 4 antibody
    • M-phase phosphoprotein 4 antibody
    • MPHOSPH4 antibody
    • MPP4 antibody
    • NF AT 90 antibody
    • NF-AT-90 antibody
    • NF110 antibody
    • NF90 antibody
    • NFAR antibody
    • Nuclear factor associated with dsRNA antibody
    • Nuclear factor of activated T cells 90 kDa antibody
    • Nuclear factor of activated T-cells 90 kDa antibody
    • TCP80 antibody
    • Translational control protein 80 antibody
    see all

Anti-ILF3 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling ILF3 with ab50832 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as teh secondary antibody. Positive staining shown in the cytoplasm and nuclei of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - ILF3. Right - Merge.
  • Anti-ILF3 antibody (ab50832) at 0.25 µg/ml + K562 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1/ 50,000 - 100,000)

    Predicted band size : 95 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    The primary antibody was diluted in 5% skim milk/PBS buffer.
  • Ab50832 (8ug/ml) staining human ILF3 in human lung tissue by immunohistochemistry using paraffin embedded tissue. Positively labelled cells, alveolar cells, are indicated with arrows.Ab50832 (8ug/ml) staining human ILF3 in human lung tissue by immunohistochemistry using paraffin embedded tissue. Positively labelled cells, alveolar cells, are indicated with arrows.

Anti-ILF3 antibody (ab50832)参考文献

This product has been referenced in:
  • Ottolini D  et al. The Parkinson disease-related protein DJ-1 counteracts mitochondrial impairment induced by the tumour suppressor protein p53 by enhancing endoplasmic reticulum-mitochondria tethering. Hum Mol Genet N/A:N/A (2013). WB ; Human . Read more (PubMed: 23418303) »
  • Guo NL  et al. Confirmation of gene expression-based prediction of survival in non-small cell lung cancer. Clin Cancer Res 14:8213-20 (2008). WB ; Human . Read more (PubMed: 19088038) »

See all 2 Publications for this product

Product Wall

DISCOUNT CODE: xxxxxxxxxx Expiration date: 19-01-2012 I am very pleased to hear you would like to accept our offer and test ab50832 in Xenopus leavis. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"