The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
Use a concentration of 2 - 3 µg/ml.
Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
Contains 1 DZF domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
ab77636 was used at 2.5µg/ml to stain ILF2 in parental and stable knock-down cell line by immunofluorescence (wildtype (left) knockdown (right). Cells were fixed with 4% formaldehyde and permeabilized with Triton X100.
Western blot - ILF2 antibody (ab77636)
Anti-ILF2 antibody (ab77636) at 0.3 µg/ml + Human Tonsil lysate in RIPA buffer at 35 µg
Predicted band size: 43 kDa Observed band size: 43 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.