Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.
Contains 1 DZF domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nuclear factor of activated T cells 45 kDa antibody
Nuclear factor of activated T-cells 45 kDa antibody
Western blot - ILF2 antibody (ab113205)
All lanes : Anti-ILF2 antibody (ab113205) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Lane 5 : NIH3T3 cell lysate at 50 µg
Predicted band size: 43 kDa
Exposure time: 10 seconds
Immunoprecipitation - ILF2 antibody (ab113205)
Detection of ILF2 by Western Blot of Immunprecipitate.
Lane 1: ab113205 at 1µg/ml staining ILF2 in HeLa whole cell lysate immunoprecipitated using ab113205 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.