Anti-IL8抗体[MM0219-7F11] (ab89336)


  • 产品名称Anti-IL8抗体[MM0219-7F11]
    参阅全部 IL8 一抗
  • 描述
    小鼠单克隆抗体[MM0219-7F11] to IL8
  • 经测试应用适用于: WB, IHC-Fr, Neutralising, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein: GAVLPRSAK ELRCQCIKTY SKPFHPKFI K ELRVIESG PH CANTEII VKL SDGREL CLDP KENWV QRVVE KFLK RAENS , corresponding to amino acids 22-98 of Human IL8



Our Abpromise guarantee covers the use of ab89336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 11 kDa.
IHC-Fr 1/20 - 1/100.
Neutralising Use at an assay dependent concentration.
Flow Cyt 1/50 - 1/200. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all

Anti-IL8 antibody [MM0219-7F11] 图像

  • Immunofluorescence analysis of Human pancreas (left panels), freshly isolated d0-islets (centre panels) and cultured d3-islets (right panels). IL8 was stained using ab89336 at 1/50 dilution.

Anti-IL8 antibody [MM0219-7F11] (ab89336)参考文献

This product has been referenced in:
  • Negi S  et al. Analysis of Beta-Cell Gene Expression Reveals Inflammatory Signaling and Evidence of Dedifferentiation following Human Islet Isolation and Culture. PLoS One 7:e30415 (2012). IHC-Fr ; Human . Read more (PubMed: 22299040) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (AGS)
Specification AGS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Aug 02 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Resin sections)
Sample Human Tissue sections (Nasal polyp and tonsil)
Specification Nasal polyp and tonsil

Abcam user community

Verified customer

提交于 Aug 05 2010

Thank you for your inquiry. I have received the following answers to your questions from the lab: 1) The actual concentration has to be determined by the end users due to the variation of cytokine concentration and cell types used. But in human...

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