RabMAb

Anti-IL8抗体[EPR1116(2)] (ab110727)

概述

  • 产品名称Anti-IL8抗体[EPR1116(2)]
    参阅全部 IL8 一抗
  • 描述
    兔单克隆抗体[EPR1116(2)] to IL8
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IL8 aa 1 to the C-terminus. The exact sequence is proprietary.

  • 阳性对照
    • WB: IL8 recombinant protein.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab110727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
  • 应用说明Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
    • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
    • 翻译后修饰Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • (Ala-IL-8)77 antibody
      • (Ser-IL-8)72 antibody
      • 9E3 antibody
      • Beta thromboglobulin like protein antibody
      • C-X-C motif chemokine 8 antibody
      • CEF-4 antibody
      • chemokine, CXC motif, ligand 8 antibody
      • CXCL8 antibody
      • Emoctakin antibody
      • GCP-1 antibody
      • GCP/IL-8 protein I antibody
      • GCP/IL-8 protein II antibody
      • GCP/IL-8 protein III antibody
      • GCP/IL-8 protein IV antibody
      • GCP/IL-8 protein V antibody
      • GCP/IL-8 protein VI antibody
      • GCP1 antibody
      • Granulocyte chemotactic protein 1 antibody
      • IL-8 antibody
      • IL-8(1-77) antibody
      • IL-8(9-77) antibody
      • IL8 antibody
      • IL8/NAP1 form I antibody
      • IL8/NAP1 form II antibody
      • IL8/NAP1 form III antibody
      • IL8/NAP1 form IV antibody
      • IL8/NAP1 form V antibody
      • IL8/NAP1 form VI antibody
      • IL8_HUMAN antibody
      • Inteleukin 8 antibody
      • LECT antibody
      • LUCT antibody
      • Lymphocyte-derived neutrophil-activating factor antibody
      • LYNAP antibody
      • MDNCF antibody
      • MDNCF-b antibody
      • MDNCF-c antibody
      • MONAP antibody
      • Monocyte-derived neutrophil chemotactic factor antibody
      • Monocyte-derived neutrophil-activating peptide antibody
      • NAF antibody
      • NAP-1 antibody
      • NAP1 antibody
      • Neutrophil activating peptide 1 antibody
      • Neutrophil activating protein 1 antibody
      • Neutrophil-activating factor antibody
      • Neutrophil-activating protein 1 antibody
      • Protein 3 10C antibody
      • Protein 3-10C antibody
      • SCYB8 antibody
      • Small inducible cytokine subfamily B member 8 antibody
      • T cell chemotactic factor antibody
      • T-cell chemotactic factor antibody
      • TSG1 antibody
      see all

    Anti-IL8 antibody [EPR1116(2)] 图像

    • Anti-IL8 antibody [EPR1116(2)] (ab110727) at 1/10000 dilution (purified) + Human interleukin 8 recombinant protein at 0.005 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 11 kDa
      Observed band size : 11 kDa

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-IL8 antibody [EPR1116(2)] (ab110727) at 1/1000 dilution (unpurified) + IL8 recombinant protein at 10 µg

      Predicted band size : 11 kDa

    Anti-IL8 antibody [EPR1116(2)] (ab110727)参考文献

    This product has been referenced in:
    • Huang WM  et al. Antioxidant and anti-inflammatory effects of Astragalus polysaccharide on EA.hy926 cells. Exp Ther Med 6:199-203 (2013). WB ; Human . Read more (PubMed: 23935746) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Human Tissue sections (Tonsil)
    Specification Tonsil
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Jun 12 2013

    Thank you for your email. This inquiry makes more sense now. Currently we do not have anti IL8 products which had been tested in sELISA in combination with other antibodies. I am sorry I am unable to recommend any capture or detection antibody at th...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"