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Our Abpromise guarantee covers the use of ab7747 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 17208399|
|WB||1/10. Detects a band of approximately 6-8 kDa (predicted molecular weight: 11.1 kDa). IL-8 appears to exist as a dimer in solution.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 25120588|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 26270987|
ab7747 staining IL8 in rat gingival tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with cold 4% paraformaldehyde. To assess the impact of P. gingivalis GroEL on the inflammatory response in gingival tissue, the expression of IL8 was assessed. As shown, there was little IL8 expression in the area of the periodontal membrane (periodontal ligament) in the control and GST treatment groups, as indicated by the black arrows. In contrast, the injection of GroEL induced significant expression of IL8 in the periodontal membrane area of the rat gingiva.
Antibody stains were developed via DAB/hydrogen peroxide reaction. Sections were subsequently counterstained with hematoxylin, dehydrated, and mounted. Finally, the slides were observed using light microscopy.
ab7747 staining IL8 in SVGA astrocytes transfected with a plasmid encoding Vpr by ICC/IF (Immunocytochemistry/immunofluorescence). The cells were stained for nucleus (blue), IL8 (green) and GFAP (red). Cells were fixed with ice-cold methanol/acetone (1:1) for 20 minutes at 20°C, washed 3x with PBS and permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at RT. Samples were incubated with ab7747 overnight in a humidified chamber. The cells were washed 5x with 0.1% Triton X-100 in PBS and incubated for 1h in secondary antibodies conjugated with Alexa Fluor 488 (Anti-Rabbit, 1:1000) at room temperature in the dark. All the antibodies were diluted in PBS containing 1% BSA.
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