概述

  • 产品名称Anti-IL8抗体[807]
    参阅全部 IL8 一抗
  • 描述
    小鼠单克隆抗体[807] to IL8
  • 特异性This antibody recognizes both, recombinant and native human IL-8. This antibody does not cross react with human Monocyte Chemotactic Activating Factor (MCAF) or RANTES (Regulated on Activation, Normal T-cell Expressed, and Secreted).
  • 经测试应用适用于: ELISA, Neutralising, WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Purified recombinant human IL-8.

  • 阳性对照

性能

应用

Our Abpromise guarantee covers the use of ab18672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.

In chemotaxis assay, the antibody inhibited 95.1% of the chemotactic activity of IL-8 on RB/293 cell. 50ug/ml of antibody and 10ng/ml of human IL-8 were used in assay. This antibody inhibited the chemotactic effect of human IL-8, and had no inhibitory effect of MIP 1-beta and RANTES in neutrophil chemotaxis assay, indicating that the neutralizing activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.

WB Use a concentration of 0.02 - 0.1 µg/ml. Predicted molecular weight: 12 kDa.

Concentration of 0.02-0.1 ug/ml of ab18672 will allow visualization of 100 ng/lane of human IL-8

IHC-Fr 1/2000.
IHC-P 1/2000.
ICC/IF Use at an assay dependent concentration. PubMed: 18697197

靶标

  • 功能IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • 序列相似性Belongs to the intercrine alpha (chemokine CxC) family.
  • 翻译后修饰Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • (Ala-IL-8)77 antibody
    • (Ser-IL-8)72 antibody
    • 9E3 antibody
    • Beta thromboglobulin like protein antibody
    • C-X-C motif chemokine 8 antibody
    • CEF-4 antibody
    • chemokine, CXC motif, ligand 8 antibody
    • CXCL8 antibody
    • Emoctakin antibody
    • GCP-1 antibody
    • GCP/IL-8 protein I antibody
    • GCP/IL-8 protein II antibody
    • GCP/IL-8 protein III antibody
    • GCP/IL-8 protein IV antibody
    • GCP/IL-8 protein V antibody
    • GCP/IL-8 protein VI antibody
    • GCP1 antibody
    • Granulocyte chemotactic protein 1 antibody
    • IL-8 antibody
    • IL-8(1-77) antibody
    • IL-8(9-77) antibody
    • IL8 antibody
    • IL8/NAP1 form I antibody
    • IL8/NAP1 form II antibody
    • IL8/NAP1 form III antibody
    • IL8/NAP1 form IV antibody
    • IL8/NAP1 form V antibody
    • IL8/NAP1 form VI antibody
    • IL8_HUMAN antibody
    • Inteleukin 8 antibody
    • LECT antibody
    • LUCT antibody
    • Lymphocyte-derived neutrophil-activating factor antibody
    • LYNAP antibody
    • MDNCF antibody
    • MDNCF-b antibody
    • MDNCF-c antibody
    • MONAP antibody
    • Monocyte-derived neutrophil chemotactic factor antibody
    • Monocyte-derived neutrophil-activating peptide antibody
    • NAF antibody
    • NAP-1 antibody
    • NAP1 antibody
    • Neutrophil activating peptide 1 antibody
    • Neutrophil activating protein 1 antibody
    • Neutrophil-activating factor antibody
    • Neutrophil-activating protein 1 antibody
    • Protein 3 10C antibody
    • Protein 3-10C antibody
    • SCYB8 antibody
    • Small inducible cytokine subfamily B member 8 antibody
    • T cell chemotactic factor antibody
    • T-cell chemotactic factor antibody
    • TSG1 antibody
    see all

Anti-IL8 antibody [807] 图像

  • ab18672 staining IL8 in porcine epithelial tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.25% Triton X-100, blocked with 10% serum for 2 hours at 25°C, then incubated with ab18672 at a 1/2000 dilution for 12 hours at 4°C. The secondary used was ab98640, used at a 1/1000 dilution.

    See Abreview

  • ab18672 staining IL8 in inflammed human temporal artery by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an antigen retrieval step was performed using sodium citrate. Samples were then blocked with 5% serum for 1 hour at 25°C, then incubated with ab18672 at a 1/2000 dilution for 16 hours at 4°C. The secondary used was a rabbit anti-mouse polyclonal conjugated to biotin, 1/1000 dilution.

    See Abreview

Anti-IL8 antibody [807] (ab18672)参考文献

This product has been referenced in:
  • Glogauer JE  et al. Neutrophils Increase Oral Squamous Cell Carcinoma Invasion through an Invadopodia-Dependent Pathway. Cancer Immunol Res 3:1218-26 (2015). Read more (PubMed: 26112922) »
  • Muthuswamy R  et al. Combination of IFNa and poly-I:C reprograms bladder cancer microenvironment for enhanced CTL attraction. J Immunother Cancer 3:6 (2015). FM ; Human . Read more (PubMed: 25806105) »

See all 4 Publications for this product

Product Wall

Application ELISA
Sample Human Recombinant protein (Human IL-8 from R&D Systems)
Specification Human IL-8 from R&D Systems
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Mar 07 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Sample Human Tissue sections (tumor)
Specification tumor
Permeabilization Yes - PBS+0.3% Triton-X-100
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 06 2013

Thank you for contacting us.
1. If you prefer to do the incubation at room temperature you should not exceed a 2 hour incubation. We do recommend an overnight incubation at 4 degrees, however, as this usually gives the strongest and most specific ...

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Thank you for your reply.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you

Credit note ID: 23605

The credit note ...

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I fully understand your concerns regarding the product code typing error on the datasheet, it is regrettable. I would like to reassure you that the image is from ab18672 IL8 antibody [807], and came from an Abreview...

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Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to offer...

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Thank you for your enquiry.

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

With regards to the text with the image, Ihave investigated thi...

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Unfortunately there is no information available about the epitope recognised by these two antibodies, as it hasn’t been mapped.

I am very sorry I couldn’t help you further. Please do not hesi...

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Thank you again for your call last week and for your patience while I have reviewed your images and these proteins.

For ab18672 (IL8), I think the diffuse bands are caused bya bittoo much proteinloaded in the lanes orfrom running the gel at t...

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Thank you very much for your call today and for letting us know about the trouble with these antibodies.

As we discussed, please send any images of the blots and we will see if there are some suggestions to improve the results. Could you also...

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