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Purified recombinant human IL-8.
Our Abpromise guarantee covers the use of ab18672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|Neutralising||Use at an assay dependent concentration.
In chemotaxis assay, the antibody inhibited 95.1% of the chemotactic activity of IL-8 on RB/293 cell. 50ug/ml of antibody and 10ng/ml of human IL-8 were used in assay. This antibody inhibited the chemotactic effect of human IL-8, and had no inhibitory effect of MIP 1-beta and RANTES in neutrophil chemotaxis assay, indicating that the neutralizing activity is specific. The antibody also neutralized IL-8 induced calcium influx in human granulocytes.
|WB||Use a concentration of 0.02 - 0.1 µg/ml. Predicted molecular weight: 12 kDa.
Concentration of 0.02-0.1 ug/ml of ab18672 will allow visualization of 100 ng/lane of human IL-8
|ICC/IF||Use at an assay dependent concentration. PubMed: 18697197|
ab18672 staining IL8 in porcine epithelial tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde, permeabilized using 0.25% Triton X-100, blocked with 10% serum for 2 hours at 25°C, then incubated with ab18672 at a 1/2000 dilution for 12 hours at 4°C. The secondary used was ab98640, used at a 1/1000 dilution.