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Recombinant full length protein corresponding to Human IL7 aa 26-177. Immunogen is the mature full length protein without the signal peptide from aa 1-25
MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH
Our Abpromise guarantee covers the use of ab84254 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 20 kDa.
To detect Human IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL7 (ab9628).
To detect Human IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. ab84254, in conjunction with ab9628 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IL-7.
ab84254 has not yet been referenced specifically in any publications.