概述

  • 产品名称Anti-IL7抗体
    参阅全部 IL7 一抗
  • 描述
    兔多克隆抗体to IL7
  • 经测试应用适用于: WB, ELISA, Neutralising, IHC-Pmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Highly pure (>98%) recombinant mIL-7 (mouse Interleukin-7)

  • 阳性对照
    • IHC-P: FFPE mouse spleen tissue sections.

性能

  • 形式Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型unknown
  • 轻链类型unknown
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab9732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-7 (using 100 µl/well antibody solution).
Neutralising Use a concentration of 0.08 - 0.1 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of mIL-7 (0.50 ng/ml).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.
  • 序列相似性Belongs to the IL-7/IL-9 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 7 antibody
    • IL-7 antibody
    • Il7 antibody
    • IL7_HUMAN antibody
    • Interleukin 7 antibody
    • Interleukin-7 antibody
    • LP-1 antibody
    • Lymphopoietin 1 antibody
    see all

Anti-IL7 antibody 图像

  • IHC image of IL7 staining in mouse spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9732, 5µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-IL7 antibody (ab9732)参考文献

This product has been referenced in:
  • Parrish YK  et al. IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrow. J Immunol 182:4255-66 (2009). Neutralising ; Mouse . Read more (PubMed: 19299724) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab9732.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"