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Recombinant full length protein corresponding to Human IL7 aa 26-177. Immunogen is the mature full length protein without the signal peptide from aa 1-25
MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH
Our Abpromise guarantee covers the use of ab9628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
To detect hIL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|Neutralising||Use a concentration of 0.04 - 0.06 µg/ml.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-7 (1.0 ng/ml), a concentration of 0.04 – 0.06 µg/ml of this antibody is required.
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL7 (Biotin) (ab84254).
To detect Human IL-7 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab84254 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-7.
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