概述

  • 产品名称
    Anti-IL6R抗体[B-R6]
    参阅全部 IL6R 一抗
  • 描述
    小鼠单克隆抗体[B-R6] to IL6R
  • 特异性
    This antibody recognizes soluble and membrane bound IL6R.
  • 经测试应用
    适用于: IHC-Fr, Blocking, Inhibition Assay, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    IL6R (gp80) transfected CHO cell line.

  • 阳性对照
    • U266 cell line

性能

应用

Our Abpromise guarantee covers the use of ab47215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. The antibody blocks IL6 induced proliferation of XG-1 cells.
Inhibition Assay Use at an assay dependent concentration. The antibody inhibits IL6 binding to its receptor.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

靶标

  • 功能
    Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
    Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.
  • 组织特异性
    Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum.
  • 序列相似性
    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 结构域
    The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  • 翻译后修饰
    A short soluble form may also be released from the membrane by proteolysis.
  • 细胞定位
    Secreted and Basolateral cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 126 antibody
    • CD126 antibody
    • CD126 antigen antibody
    • gp80 antibody
    • IL 6R 1 antibody
    • IL 6R alpha antibody
    • IL 6R antibody
    • IL-6 receptor alpha chain antibody
    • IL-6 receptor subunit alpha antibody
    • IL-6R 1 antibody
    • IL-6R subunit alpha antibody
    • IL-6R-alpha antibody
    • IL-6RA antibody
    • IL6Q antibody
    • Il6r antibody
    • IL6RA antibody
    • IL6RA_HUMAN antibody
    • IL6RQ antibody
    • Interleukin 6 receptor antibody
    • interleukin 6 receptor, alpha antibody
    • Interleukin-6 receptor subunit alpha antibody
    • Membrane glycoprotein 80 antibody
    • MGC30256 antibody
    see all

图片

  • ab47215 staining IL6R on U266 cell line.

文献

This product has been referenced in:
  • Mi S  et al. Microfluidic co-culture system for cancer migratory analysis and anti-metastatic drugs screening. Sci Rep 6:35544 (2016). ICC/IF ; Human . Read more (PubMed: 27762336) »
  • He YY  et al. Establishment of a novel cell-based assay for screening small molecule antagonists of human interleukin-6 receptor. Acta Pharmacol Sin 35:1453-62 (2014). ELISA . Read more (PubMed: 25345743) »

See all 5 Publications for this product

客户评价及客户问答



I have added the protocol the lab uses to the end of this email for you. Please note that the experiment is done radioactively with Thymidine H3.

Regarding the amount of IL-6 found in the serum, 15pg/ml, it is 60 time less than the a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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