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Our Abpromise guarantee covers the use of ab84251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 24 kDa.|
|Sandwich ELISA||Use at an assay dependent concentration. To detect hIL-6 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 – 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Mouse monoclonal to IL6 (ab9324) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-6.|
ab84251 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"