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Our Abpromise guarantee covers the use of ab114982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse IL5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use a concentration of 0.25 - 1 µg/ml. ab114982, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IL5.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml. ab114982, in conjunction with a Polyclonal Anti-Mouse IL5 as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse IL5.|
ab114982 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"