Anti-IL-4R (phospho Y497)抗体(ab61099)
Key features and details
- Rabbit polyclonal to IL-4R (phospho Y497)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IL-4R (phospho Y497)抗体
参阅全部 IL-4R 一抗 -
描述
兔多克隆抗体to IL-4R (phospho Y497) -
宿主
Rabbit -
特异性
Detects endogenous levels of IL4R only when phosphorylated at tyrosine 497 (corresponding to mouse tyrosine 500) -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide corresponding to Human IL-4R aa 450-550 (phospho Y497).
Database link: P24394 -
阳性对照
- 293 and HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit antiserum by affinity chromatography, using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61099于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
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ICC/IF |
1/500 - 1/1000.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells. -
组织特异性
Isoform 1 and isoform 2 are highly expressed in activated T-cells. -
序列相似性
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain. -
结构域
The extracellular domain represents the IL4 binding protein (IL4BP).
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
翻译后修饰
On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3566 Human
- Entrez Gene: 16190 Mouse
- Omim: 147781 Human
- SwissProt: P24394 Human
- SwissProt: P16382 Mouse
- Unigene: 513457 Human
- Unigene: 233802 Mouse
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别名
- CD124 antibody
- IL 4R alpha antibody
- IL-4 receptor subunit alpha antibody
see all
图片
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All lanes : Anti-IL-4R (phospho Y497) antibody (ab61099) at 1/500 dilution
Lane 1 : Extracts from 293 cells
Lane 2 : Extracts from 293 cells with immunizing phosphopeptide
Predicted band size: 89 kDa
Observed band size: 89 kDa -
ICC/IF image of ab61099 (at a 1/500 dilution) stained HeLa cells, minus immunising peptide (left image) and plus immunising peptide (right image).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab61099 被引用在 1 文献中.
- Kurgonaite K et al. Essential role of endocytosis for interleukin-4-receptor-mediated JAK/STAT signalling. J Cell Sci 128:3781-95 (2015). PubMed: 26306492