Customer reviews and FAQs

概述

  • 产品名称
    Anti-IL4I1抗体
    参阅全部 IL4I1 一抗
  • 描述
    兔多克隆抗体to IL4I1
  • 经测试应用
    适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human IL4I1.

    参阅Abcam的专有抗源政策

    (Peptide available as ab19824.)

  • 阳性对照
    • WB: Human testis tissue lysate. ICC/IF: HeLa cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab18524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 19436310
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).

靶标

  • 相关性
    IL-4-induced gene-1 (Il4i1 or Fig1) is expressed in primarily immune tissues and genetically maps to a region of susceptibility to autoimmune disease. The predicted Il4i1 protein (IL4I1) sequence is most similar to apoptosis-inducing protein and Apoxin I, which are both l-amino acid oxidases.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
    • 别名
      • FIG 1 antibody
      • Fig 1 protein antibody
      • FIG1 antibody
      • Fig1 protein antibody
      • HCG2043070 antibody
      • hFIG 1 antibody
      • hFIG1 antibody
      • IL4 induced protein 1 antibody
      • Interleukin 4 induced 1 antibody
      • Interleukin 4 induced protein 1 antibody
      • Interleukin four induced 1 antibody
      • L amino acid oxidase antibody
      • LAAO antibody
      • LAO antibody
      • Protein Fig 1 antibody
      • Protein Fig1 antibody
      see all

    Anti-IL4I1 antibody 图像

    • Western blot - Anti-IL4I1 antibody (ab18524)
      Western blot - Anti-IL4I1 antibody (ab18524)
      Anti-IL4I1 antibody (ab18524) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 63 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

       

      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab18524 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    • Immunocytochemistry/ Immunofluorescence - IL4I1 antibody (ab18524)
      Immunocytochemistry/ Immunofluorescence - IL4I1 antibody (ab18524)

      ICC/IF image of ab18524 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in paraformaldehyde for 10 minutes, permabilized in TBS-T (20 minutes) and incubated with the antibody (ab18524, 5 µg/ml) for 1 hour at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to quench auto-fluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

    Anti-IL4I1 antibody (ab18524)参考文献

    This product has been referenced in:
    • Marquet J  et al. Dichotomy between factors inducing the immunosuppressive enzyme IL-4-induced gene 1 (IL4I1) in B lymphocytes and mononuclear phagocytes. Eur J Immunol 40:2557-68 (2010). Read more (PubMed: 20683900) »
    • Carbonnelle-Puscian A  et al. The novel immunosuppressive enzyme IL4I1 is expressed by neoplastic cells of several B-cell lymphomas and by tumor-associated macrophages. Leukemia 23:952-60 (2009). IHC-P ; Human . Read more (PubMed: 19436310) »

    See all 3 Publications for this product

    发表研究结果有使用 ab18524？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Western blot abreview for Anti-IL4I1 antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (BMDM)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    BMDM
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Abcam user community

    Verified customer

    提交于 Oct 10 2016

    Product code: 18524
    Lot number: 149276
    Inquiry: This product ( polyclonal antibody anti-IL4I1) has been unavailable for a long time. Would it be possible to know the reason and know when and if it will be available again? I asked this questio...

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    Thank you for contacting us and sorry for the delay in getting back to you.

    I have looked into the reason why the Anti-IL4I1 antibody (ab18524) has been unavailable for some time now. Unfortunately, we have been trying to produce new batches ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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