Anti-IL3RB (phospho Y593)抗体(ab53695)

概述

  • 产品名称Anti-IL3RB (phospho Y593)抗体
    参阅全部 IL3RB 一抗
  • 描述
    兔多克隆抗体to IL3RB (phospho Y593)
  • 特异性ab53695 detects endogenous levels of IL3RB only when phosphorylated at tyrosine 593.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human IL3RB around the phosphorylation site of tyrosine 593 (G-P-YP-L-G).

  • 阳性对照
    • Human brain tissue and RAW264.7 cell extracts treated with G-CSF (25ng/ml, 15mins).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab53695 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab53695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 97 kDa.

靶标

  • 功能High affinity receptor for interleukin-3, interleukin-5 and granulocyte-macrophage colony-stimulating factor.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 4 subfamily.
    Contains 2 fibronectin type-III domains.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • /granulocyte-macrophage colony-stimulating factor receptor, beta antibody
    • Beta-C antibody
    • CD131 antibody
    • CDw131 antibody
    • Colony stimulating factor 2 receptor beta antibody
    • Colony stimulating factor 2 receptor beta low affinity (granulocyte macrophage) antibody
    • Common beta chain antibody
    • Csf2rb antibody
    • Cytokine receptor common beta chain antibody
    • Cytokine receptor common beta chain precursor (CDw131 antigen) antibody
    • Cytokine receptor common subunit beta antibody
    • GM CSF/IL 3/IL 5 receptor common beta chain antibody
    • GM-CSF/IL-3/IL-5 receptor common beta subunit antibody
    • IL3RB antibody
    • IL3RB_HUMAN antibody
    • IL5RB antibody
    • Interleukin 3 receptor, beta antibody
    • Interleukin 3 receptor/granulocyte macrophage colony stimulating factor 3 receptor beta (high affinity) antibody
    • Interleukin 5 receptor, beta antibody
    • SMDP5 antibody
    • stimulating factor 3 receptor, beta (high affinity) antibody
    see all

Anti-IL3RB (phospho Y593) antibody 图像

  • ab53695 at 1/50 dilution staining IL3RB in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-IL3RB (phospho Y593) antibody (ab53695)

    Lane 1 : HepG2 cells treated with EGF (200ng/ml, 15 minutes)
    Lane 2 : HepG2 cells treated with EGF (200ng/ml, 15 minutes) with blocking phosphopeptide


    Predicted band size : 97 kDa

Anti-IL3RB (phospho Y593) antibody (ab53695)参考文献

ab53695 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab53695.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"