The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells.
Use 0.5µg for 106 cells in 100µl or 100µl whole blood. This antibody has been tested using immunofluorescent staining with flow cytometric analysis.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
This is a receptor for interleukin-3.
Belongs to the type I cytokine receptor family. Type 5 subfamily.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Sun Q et al. Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist. Blood87:83-92 (1996).
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